Welcome
to the Growing tips section of the North Florida Shroom Guide. Here you will
find modifications to grow instructions available in Cowboy's Grow Room as
well as how to do things not listed in the various instructions.
You may skip directly to the section of your interest by clicking on the links
below, or just scroll down to browse the entire page.
|
|
Syringe Diluting | Casing | Bedding |
| Rye Grain Method | Alternative Fruiting Chamber | Making Spore Syringes |
After having little success in making a sterile spore
syringe I added another step to decrease contamination.
Proceed
with taking a spore print in the manner described in the mmgg.
Sterilize many syringes including a larger (25-50cc) syringe. Draw sterilized
water into all syringes. Open your half pint jar with your print in it and
use your big syringe to fill the jar half way without trying to disturb the
spores. Now you should have a jar half full of water with the spores still
stuck to the bottom.
Now take your other syringes and penetrate the top layer of water and while
keeping the needle under the water inject about a half cc of water and scrape
a little of the spores off the bottom and suck them in. Be sure to keep the
needle under water the whole time. Repeat this step until you are all finished.
Discard the rest of the print and the half jar of water.
This method works well because any contaminants that may come into play after
you open the jar will float on top of the water and all the work is done under
the water.
sent to us by mycelium5150.
(I WILL NOT OFFER TECH SUPPORT ON TRYPTAMINE GROWTH)
First off, the PF tryptamine cubensis variation works very well,except that
the main head-wriggling compound produced is psilocin as opposed to the more
shelf stable psilocybin.
As many may have already figured out, there just isn't enough phosphorus in
the substrate to support psilocybin production...quite simply, it is used
up. My answer to this was to add a small amount of phosphoric acid (H3PO4)to
the water at the same time you add the tryptamine HCl, and adjust to neutral
to wide-base Ph paper using NaOH.
I admit to a small amount of salt formation, but I haven't seen adverse results
from the tiny bit of NaCl produced. A reminder is that many of the best hunting
places in Fla. and Texas are saltmarsh and near-coastal lowlands.
I figure the organism is already accustomed to a small amount of salts in
it's diet. This will give the mushie substrate enough Phosphorus to complete
the conversion of psilocin to psilocybin in vivo. Hence, when you dry and
store your mushrooms properly, the more stable psilocybin molecule is retained
as opposed to psilocin, which oxidizes rather quickly.
THANKS TO SUNSHINE MIKE FOR THIS ONE!!!!
from trevorc:
I have some tips for you on that method.
1- you must pressure cook the jars of rye grain. Boiling them just does not
work to sterilize. I have found that out the hard way and so have you. I did
not believe this when someone told me but I tried it anyways and it work 90%
successes rate. I only lost one out of every Ten jars. I found I got contamination
blue green mold and a slimy light brown sludge on the culture when just boiling
them also slow slow growth.
Try to pressure cook it and you will have success also add soil conditioner*
to your mixture of ryegrain and water this sets the ph properly
(* could use crushed chalk, oyster shell or the easy thing to find is calcium
carbonate 1/4 teaspoon or a large pinch).
The rye is the food for the mycelium to feed on and when the Ph is proper
the food is easier to digest thus speeding growth. like our stomachs. This
does not speed it dramatically but will help.
Also the amount of light given during culturing affects it. The best thing
for its is complete darkness and a temp of about 76-82 degrees these are the
determining factors in how well and fast it grows.
Now when you have a couple %100 cultured rye grain jars. you can use them
to make 1000s more. Take one jar and shake it up to loosen the mycelium so
there are just white colored loose rye grains in the jar no clumps let sit
for 14 hours to let the mycelium wake up or regain growth.
Now prepare 8-10 more uncultured jars by using the mixture of
1
cup-rye
2/3-3/4cup-water
1/4 teaspoon chalk or calcium carbonate.
pressure cook them and let them cool for 3 hours then in a sterile transfer
chamber(make one) put the 8 jars in plus the fully cultured jar.
Now un cap the cultured jar and dump 1/10th of the mycelium into the uncultured
jar and close the lid, repeat for all ten jars. Then shake up those jars to
spread the mycelium though the uncultured jar.
Let stand in its climate (dark and warm) for 4 days you will see hard-core
growth about %50 of the jar will be cultured
you can shake the jar again to spread the mycelium evenly throughout the uncultured
areas.
In about another 4-5 days the jar will be fully cultured.
This method really works and you don't have to keep using the spores as they
take longer to culture and are hard to collect without causing contamination
to them. By using this method you cut out one step in a three step procedure-
Steps are
1-spore germination produces
2-mycilum production which produces
3-fruiting or mushroom growth.
Now you only ever have to 2 produce mycelium and 3 induce fruiting. If you
have any questions on this or another question feel free to ask me my E-mail
is Trevorc@rocketmail.com
Spore
Syringe Diluting!!
from guitar9
Here is a tip I learned from being a cheap bastard.
I first started paying ten dollars for the pf syringes and it calls for 1
cc per jar.
I noticed that was far more than was needed.I tried diluting 1 pf syringe
into a pint of sterilized water(spring water).That gives you the opportunity
to inject more solution to cover more surface area thus shorting your incubation
time and you get 20-30 syringes that way(i cant tell a difference between
diluted and undiluted!)Keep the solution in the fridge for storage.I ran into
a problem with contamination when you use them for a long time when you use
a lid with a hole.I solved this problem by purchasing a filter disc for the
top of my pint jar,when you extract the solution,unwanted air and contaminates
want to enter due to equalization of air pressure.If you use a filter and
flame sterilize the syringe and burn a perfect size hole for the needle you
wont have this problem,just remember to put a piece of tapeover the hole.Pf
instructions for spore solution did not address this problem.Also use petri
dishes for spore prints and burn perfect hole in the side an inject water
,extract,and dilute.Also works for mycelium cultures.I could never afford
hepa filters so I had to use what i had,now im glad!!:-)i have grown many
wild strains this way too!!! Thanks NFSG you brought a tear to my eye when
I found you!!!!
CASING
Here
is a tip from a reader about what to do with you cakes after they are almost
done fruiting: It is called Casing!!! And sounds like a damn smart idea. EnJoy!!!!!!!!!
Take some Tupperware trays about 6" by 10" and crumple up a few of the cakes
when they are at or close to their max age. Throw seran wrap over this and
let it grow for about 3 days. Then case it with moist vermiculite and again
cover with seran wrap. Leave this in a warm place but not necessarily in the
terrarium. The little kids will be growing in no time. When most of the surface
of the vermiculite looks about colonized take off the wrap and put it into
the terrarium.
from the So. Cal. 421 Kids............
i tried breaking up3 dead cakes in a tupper ware bowl about 5 in.x5in. i broke
them up andspread the cakes all along the bottom of the tupperware, then i
wrapped the tupperware in seran wrap.i left the Tupperware in a dark place.By
doing so it helps the mycelium to start togrownew fuzz.about 4 days later
i took out the Tupperware and unwrapped it.then i got some damp vermiculite,{not
muddy;just moist} and made a thin layer over the fuzzier cakes.i wrapped it
again and left alone for about 7 days to let the mycelium grow more.after
that i put the Tupperware in my terrarium.now about 6 days later of hand spraying
and perlite i have fresh, brand new ,home boys coming in.just make sure you
give the cakes time to re-grow the mycelium!
Fruiting Chamber From Toad B Shrewman
I have been following methods of cake growers for a couple years and most
seem to be using aquariums or coolers. Here is what I have always used and
it's time I posted it so others can try it too. I "think" it's somewhat original
so please pass it on.
If you go to Home Depot or a large hardware center, go to the plumbing area
and look for the plastic laundry sinks. There is one made of a PVC material
and is about $20. Here is a description: White plastic sink comes with four
detachable legs-when assembled stands 2ft 8in tall, the sink chamber is about
12in deep with a 20x20in square opening on top that has a 3/16th inch high
lip or rim running around it. The opening MUST have this rim. The bottom of
the sink chamber has(of course)a drain hole in the bottom.(see where im going
with this?)
Now, get the sink and take an EXACT measurement of the opening INSIDE the
rim on top(should be around 21 1/2 x 22 3/8 inches square)then have a piece
of glass cut to that size, line the edges of the glass with duct tape and
you have your chamber lid. The duct tape acts to seal the chamber cuz the
rubberized tape surface is resting on the plastic sink while the glass square
is lying inside the rim of the sink. If you dont see a place to lay the glass
or the opening comes straight up with no rim, you gots da wrong sink(look
for this rim).
Use the method of your choice to keep the cakes off the bottom. If you do
not use a bubbler jar you can fit about 12 cakes in the bottom. The drain
in the bottom is self explanatory and you can get a plug to fit it or rig
up an external valve on the outside pipe so you dont have to open the chamber
to drain it(no more tilting coolers or turkey basting aquariums):-). The plastic
sink is very easy to drill into so measure the area for your drip shield,
get the glass and hold it up with some screws or something pushed through
the sides. If you use the ultrasonic just cut the needed holes.
My sink method can't be EXACTLY instructed cuz every individual has different
preferences.
ADVANTAGES ARE: Good fitting glass lid-(no making a frame with plastic).
Elevated chamber-(stands on its own legs).
Drain built in-(obvious advantage).
Fits up to 12 rice cakes-
Adapts to your method-(drill and cut holes with more variety).
Hide your project quick-(is grandma dropping by? Just throw a sheet over the
thing, set a vase and a bowl of plastic fruit on top, cuz its waist high like
a table).
SEE GUYS AND ESPECIALLY GALS, IT IS YOUR SHROOM GUIDE GIVE ME SOME INTELLIGENT SHIT AND IT GETS POSTED!!!!!
CLICK
HERE to Go Back to the Grow Room
CLICK
HERE to Go Back to the Main North Florida Shroom
Guide Page